<?xml:namespace prefix = o ns = "urn:schemas-microsoft-com:office:office" />

Experimental study about dexamethasone to the proliferation of Rat aortic smooth muscle cells

王建波¹，杨建勇¹，陈伟¹，庄文权¹，张泷娟²

Jianbo Wang¹，Jianyong Yang¹，Wei Chen¹，Wenquan Zhuang¹，Longjuan Zhang²

（1.Department of Interventional Radiology，2.Laboratory of Surgery，The First Affiliated Hospital，SUN Yat－sen University，Guangzhou 510080，China）

（中山大学第一附属医院  1 放射介入科；2 外科实验室 510080）

Abstract

　　Objective：To evaluate the effect of dexamethasone to the cultured Rat thoracic aortic smooth muscle cells（SMC） in vitro.

　　Method：The Rat thoracic aortic SMC were harvest and cultured for six to ten passages. Cultured SMC were synchronized，then restimulated to enter the cell cycle，and treated with incremental concentrations of dexamethasone or without dexamethasone as contrast. The proliferative assay was performed with MTT method in the different time points after treated. DNA synthesis was determined 48 hours after restimulation by measuring [3H] thymidine incorporation. RT－PCR was performed to assay the level of proliferating cell nuclear antigen （PCNA） mRNA.